Question 1

TATAAT is a consensus sequence located at -10 upstream of the transcription start site.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following compounds is effective in inhibiting RNA chain polymerization in an in vitro transcription assay?

Accepted Answer

A) treptomycin 
B) ampicillin 
C) streptolydigin 
D) treptomycin and ampicillin 
E) ampicillin and streptolydigin 
A) treptomycin 
B) ampicillin 
C) streptolydigin 
D) treptomycin and ampicillin 
E) ampicillin and streptolydigin

Question 3

An experiment was conducted to determine the hairpin loop region in termination.Two DNA templates were prepared and used in an in vitro transcription assay.The samples were analyzed for release of the RNA polymerase.Construct 1 - hairpin sequence removed; Construct 2 - two copies of hairpin sequence inserted; Construct 3 - normal DNA with only one hairpin sequence   The data from this experiment suggest that

Accepted Answer

A) hairpin loops are not required for termination. 
B) two hairpin loops rapidly destabilize the polymerase. 
C) the data are inconclusive. 
D) additional hairpin loops increase the stability of the RNA:DNA hybrid. 
E) both hairpin loops are not required for termination and two hairpin loops rapidly destabilize the polymerase are possible explanations. 
A) hairpin loops are not required for termination. 
B) two hairpin loops rapidly destabilize the polymerase. 
C) the data are inconclusive. 
D) additional hairpin loops increase the stability of the RNA:DNA hybrid. 
E) both hairpin loops are not required for termination and two hairpin loops rapidly destabilize the polymerase are possible explanations.

Question 4

Which of the following is most involved in elongation of the RNA transcript?

Accepted Answer

A) sigma factor 
B) UP elements 
C) -10 region 
D) both sigma factor and UP elements 
A) sigma factor 
B) UP elements 
C) -10 region 
D) both sigma factor and UP elements

Question 5

Dimethyl sulfate is used to

Accepted Answer

A) cleave DNA molecules. 
B) denature or melt DNA promote region. 
C) remove methyl groups from RNA. 
D) add methyl groups to DNA. 
E) promote the binding of RNA polymerase to DNA. 
A) cleave DNA molecules. 
B) denature or melt DNA promote region. 
C) remove methyl groups from RNA. 
D) add methyl groups to DNA. 
E) promote the binding of RNA polymerase to DNA.

Question 6

Which of the following factors is involved in proofreading during transcription?

Accepted Answer

A) GreB 
B) NusA 
C) sigma-factor 
D) rho-factor 
E) none of the choices are correct. 
A) GreB 
B) NusA 
C) sigma-factor 
D) rho-factor 
E) none of the choices are correct.

Question 7

Which of the following is true about the holoenzyme in an open complex?

Accepted Answer

A) The DNA is bound mainly to the sigma-subunit. 
B) There no interaction between the sigma factor and the -10 region. 
C) There are two Na+ ions in the core enzyme . 
D) Region 2.4 of the sigma-factor binds to the -35 region. 
E) The DNA is bound mainly to the sigma-subunit and there no interaction between the sigma factor and the -10 region are both true. 
A) The DNA is bound mainly to the sigma-subunit. 
B) There no interaction between the sigma factor and the -10 region. 
C) There are two Na+ ions in the core enzyme . 
D) Region 2.4 of the sigma-factor binds to the -35 region. 
E) The DNA is bound mainly to the sigma-subunit and there no interaction between the sigma factor and the -10 region are both true.

TATAAT is a consensus sequence located at -10 upstream of the transcription start site.

Which of the following compounds is effective in inhibiting RNA chain polymerization in an in vitro transcription assay?

Which of the following is most involved in elongation of the RNA transcript?

Dimethyl sulfate is used to

Which of the following factors is involved in proofreading during transcription?

Which of the following is true about the holoenzyme in an open complex?

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

Exam 6: The Mechanism of Transcription in Bacteria

TATAAT is a consensus sequence located at -10 upstream of the transcription start site.

Which of the following compounds is effective in inhibiting RNA chain polymerization in an in vitro transcription assay?

Which of the following is most involved in elongation of the RNA transcript?

Dimethyl sulfate is used to

Which of the following factors is involved in proofreading during transcription?

Which of the following is true about the holoenzyme in an open complex?

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters