Exam 20: Dna Tools and Biotechnology

Let us suppose that someone is successful at producing induced pluripotent stem cells (iPS)for replacement of pancreatic insulin-producing cells for people with type 1 diabetes. Which of the following could still be problems? I. the possibility that, once introduced into the patient, the iPS cells produce nonpancreatic cells II. the failure of the iPS cells to take up residence in the pancreas III. the inability of the iPS cells to respond to appropriate regulatory signals

Use the following information to answer the next few questions. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. -Bacteria that contain the plasmid, but not the eukaryotic gene, would grow

RNAi methodology uses double-stranded pieces of RNA to trigger a breakdown or blocking of mRNA. For which of the following might it more possibly be useful?

Scientists developed a set of guidelines to address the safety of DNA technology. Which of the following is one of the adopted safety measures?

Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

Silencing of selected genes is often done using RNA interference (RNAi). Which of the following questions would not be answered with this process?

Use the following information to answer the next few questions. A group of six students has taken samples of their own cheek cells, purified the DNA, and used a restriction enzyme known to cut at zero, one, or two sites in a particular gene of interest. -Analysis of the data obtained shows that two students each have two fragments, two students each have three fragments, and two students each have one only. What does this demonstrate?

Reproductive cloning of human embryos is generally considered unethical. However, on the subject of therapeutic cloning there is a wider divergence of opinion. Which of the following is a likely explanation?

As genetic technology makes testing for a wide variety of genotypes possible, which of the following is likely to be an increasingly troublesome issue?

An unspecialized cell that can reproduce and differentiate is a(n)________ cell.

Which of the following is most closely identical to the formation of twins?

Use the following figure to answer the questions below. The DNA profiles that follow represent four different individuals. -Which of the following statements is consistent with the results?