Exam 14: Dna Structure, Replication, and Organization

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The T2 bacteriophages used in the Hershey and Chase experiment contain ____.

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Suppose that you performed a version of the Hershey and Chase experiment, this time using32P-labeled viruses that insert their double-stranded DNA into the DNA of the cells that they infect. The viral DNA is then treated as part of the cell's own DNA and is replicated during DNA replication and passed onto daughter cells when the cell divides. You infect a population of cells with the32P-labeled viruses, and then let the infected cells go through two generations of cell divisions. If you then examine the cells, you should find32P-labeled DNA in ____ of the cells.

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The steps of DNA replication and the enzymes involved are largely conserved amongst bacteria, archaea, and eukaryotes. How can this evolutionary conservation be explained?

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Some research has indicated that increased exercise is linked to increased telomere length in renewable tissues, like blood. Assuming that telomerase levels are not increased by exercise, what might explain the increase in telomere length?

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Prior to the 1940s, many biologists believed ____, which is composed of 20 different types of ____, was most likely the genetic material.

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The alteration of a cell's hereditary type by the uptake of DNA released by the breakdown of another cell is called ____.

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What is unlikely to happen if the sliding clamp is nonfunctional?

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Adjacent nucleotides on a strand of DNA are connected to each other by a(n) ____.

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What happens when living R strain Streptococcus pneumoniae bacteria are mixed with heat-killed S strain Streptococcus pneumoniae bacteria?

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A DNA double helix has two strands that are held together by ____.

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The two strands of a DNA double helix are said to be antiparallel. This means that ____.

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Classification
For the following questions, match the enzyme involved in DNA replication with its function.
Tethers DNA polymerase to the template DNA
DNA polymerase I
Main enzyme that extends RNA primer by adding DNA nucleotides to it
DNA ligase
Unwinds DNA helix
sliding clamp
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Responses:
Tethers DNA polymerase to the template DNA
DNA polymerase I
Main enzyme that extends RNA primer by adding DNA nucleotides to it
DNA ligase
Unwinds DNA helix
sliding clamp
Stabilizes single-stranded DNA
primase
Relieves over-winding of DNA ahead of the replication fork
single-stranded binding proteins
Synthesizes short RNA segment to initiate new DNA strand
DNA polymerase III
Contains 5' ->3' exonuclease activity to remove RNA preceding previous Okazaki fragment and uses its 5' ->3' polymerization activity to replace RNA nucleotides with DNA nucleotides
helicase
Seals nicks between adjacent DNA segments
topoisomerase
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Wilkins and Franklin studied the structure of DNA using ____.

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After DNA repair enzymes remove an incorrect nucleotide resulting from a replication error, ____ is/are needed to complete the repair.

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Avery, MacLeod, and McCarty showed that DNA was the transforming principle by demonstrating that mice survived when injected with a mixture of dead virulent bacteria and live nonvirulent bacteria that were treated with a DNA degrading enzyme. If RNA was the genetic material, what would have been the effect on the mice? Why?

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In many cancers, the cancerous cells have fully active telomerase enzymes. Explain how this might play a role in enabling cancer cells to keep rapidly dividing.

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Cancer treatments may specifically target rapidly dividing cells by using drugs or chemicals that ____.

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Which statement correctly describes DNA base pairing?

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In DNA, the pyrimidines are ____.

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Individuals with xeroderma pigmentosum inherit a faulty DNA repair mechanism. As a consequence, ____.

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