Exam 27: Bacteria and Archaea

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During which two times can the recipient accurately be described as "recombinant" due to the sequence of events portrayed in Figure 27.2?

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Regarding prokaryotic genetics, which statement is correct?

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The following questions refer to Figure 27.1. In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population. The following questions refer to Figure 27.1. In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population.     Figure 27.1 -Which term best describes what has occurred among the experimental populations of cells over this eight-year period? Figure 27.1 -Which term best describes what has occurred among the experimental populations of cells over this eight-year period?

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Nitrogenase, the enzyme that catalyzes nitrogen fixation, is inhibited whenever free O₂ reaches a critical concentration. Consequently, nitrogen fixation cannot occur in cells wherein photosynthesis produces free O₂. Consider the colonial aquatic cyanobacterium, Anabaena, whose heterocytes are described as having "…a thickened cell wall that restricts entry of O₂ produced by neighboring cells. Intracellular connections allow heterocysts to transport fixed nitrogen to neighboring cells in exchange for carbohydrates." -Given that the enzymes that catalyze nitrogen fixation are inhibited by oxygen, what are two "strategies" that nitrogen-fixing prokaryotes might use to protect these enzymes from oxygen? 1)couple them with photosystem II (the photosystem that splits water molecules) 2)package them in membranes that are impermeable to all gases 3)be obligate anaerobes 4)be strict aerobes 5)package these enzymes in specialized cells or compartments that inhibit oxygen entry

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Use the information in the following paragraph to answer the next few questions. A hypothetical bacterium swims among human intestinal contents until it finds a suitable location on the intestinal lining. It adheres to the intestinal lining using a feature that also protects it from phagocytes, bacteriophages, and dehydration. Fecal matter from a human in whose intestine this bacterium lives can spread the bacterium, even after being mixed with water and boiled. The bacterium is not susceptible to the penicillin family of antibiotics. It contains no plasmids and relatively little peptidoglycan. -The cell also lacks F factors and F plasmids. Upon its death, this bacterium should be able to participate in

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Assuming that each of these possesses a cell wall, which prokaryotes should be expected to be most strongly resistant to plasmolysis in hypertonic environments?

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Carl Woese and collaborators identified two major branches of prokaryotic evolution. What was the basis for dividing prokaryotes into two domains?

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Which of the following is least associated with the others?

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Which of the following traits do archaeans and bacteria share? 1)composition of the cell wall 2)presence of plasma membrane 3)lack of a nuclear envelope 4)identical rRNA sequences

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Nitrogenase, the enzyme that catalyzes nitrogen fixation, is inhibited whenever free O₂ reaches a critical concentration. Consequently, nitrogen fixation cannot occur in cells wherein photosynthesis produces free O₂. Consider the colonial aquatic cyanobacterium, Anabaena, whose heterocytes are described as having "…a thickened cell wall that restricts entry of O₂ produced by neighboring cells. Intracellular connections allow heterocysts to transport fixed nitrogen to neighboring cells in exchange for carbohydrates." -Which two questions below arise from a careful reading of this quotation, and are most important for understanding how N₂ enters heterocysts, and how O₂ is kept out of heterocysts? 1)If carbohydrates can enter the heterocysts from neighboring cells via the "intracellular connections," how is it that O₂ doesn't also enter via this route? 2)If the cell walls of Anabaena's photosynthetic cells are permeable to O₂ and CO₂, are they also permeable to N₂? 3)If the nuclei of the photosynthetic cells contain the genes that code for nitrogen fixation, how can these cells fail to perform nitrogen fixation? 4)If the nuclei of the heterocysts contain the genes that code for photosynthesis, how can these cells fail to perform photosynthesis? 5)If the cell walls of Anabaena's heterocysts are permeable to N₂, how is it that N₂ doesn't diffuse out of the heterocysts before it can be fixed? 6)If the thick cell walls of the heterocysts exclude entry of oxygen gas, how is it that they don't also exclude the entry of nitrogen gas?

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The data were collected from the heterocysts of a nitrogen-fixing cyanobacterium inhabiting equatorial ponds. Study the following graph and choose the most likely explanation for the shape of the curve. The data were collected from the heterocysts of a nitrogen-fixing cyanobacterium inhabiting equatorial ponds. Study the following graph and choose the most likely explanation for the shape of the curve.

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Chloramphenicol is an antibiotic that targets prokaryotic (70S)ribosomes, but not eukaryotic (80S)ribosomes. Which of these questions stems from this observation, plus an understanding of eukaryotic origins?

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In Fred Griffith's experiments, harmless R strain pneumococcus became lethal S strain pneumococcus as the result of which of the following? 1)horizontal gene transfer 2)transduction 3)conjugation 4)transformation 5)genetic recombination

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If archaeans are more closely related to eukaryotes than to bacteria, then which of the following is a reasonable prediction?

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Use the information in the following paragraph to answer the next few questions. A hypothetical bacterium swims among human intestinal contents until it finds a suitable location on the intestinal lining. It adheres to the intestinal lining using a feature that also protects it from phagocytes, bacteriophages, and dehydration. Fecal matter from a human in whose intestine this bacterium lives can spread the bacterium, even after being mixed with water and boiled. The bacterium is not susceptible to the penicillin family of antibiotics. It contains no plasmids and relatively little peptidoglycan. -This bacterium's ability to survive in a human who is taking penicillin pills may be due to the presence of 1)penicillin-resistance genes 2)a secretory system that removes penicillin from the cell 3)a gram-positive cell wall 4)a gram-negative cell wall 5)an endospore

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The following questions refer to Figure 27.1. In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population. The following questions refer to Figure 27.1. In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population.     Figure 27.1 -Which of the following, if it occurs in the absence of any other type of adaptation listed here, is least reasonable in terms of promoting bacterial survival over evolutionary time in a low-glucose environment? Figure 27.1 -Which of the following, if it occurs in the absence of any other type of adaptation listed here, is least reasonable in terms of promoting bacterial survival over evolutionary time in a low-glucose environment?

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The following table depicts characteristics of five prokaryotic species (A-E). Use the information in the table to answer the following questions. The following table depicts characteristics of five prokaryotic species (A-E). Use the information in the table to answer the following questions.    -Which two species might be expected to cooperate metabolically, perhaps forming a biofilm wherein one species surrounds cells of the other species? -Which two species might be expected to cooperate metabolically, perhaps forming a biofilm wherein one species surrounds cells of the other species?

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The thermoacidophile, Sulfolobus acidocaldarius, lacks peptidoglycan, but still possesses a cell wall. What is likely to be true of this species? 1)It is a bacterium. 2)It is an archaean. 3)The optimal pH of its enzymes will lie above pH 7. 4)The optimal pH of its enzymes will lie below pH 7. 5)It could inhabit certain hydrothermal springs. 6)It could inhabit alkaline hot springs.

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Which statement about the domain Archaea is true?

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In a hypothetical situation, a bacterium lives on the surface of a leaf, where it obtains nutrition from the leaf's nonliving, waxy covering while inhibiting the growth of other microbes that are plant pathogens. If this bacterium gains access to the inside of a leaf, however, it causes a fatal disease in the plant. Once the plant dies, the bacterium and its offspring decompose the plant. What is the correct sequence of ecological roles played by the bacterium in the situation described here? Use only those that apply. 1)nutrient recycler 2)mutualist 3)commensal 4)parasite 5)primary producer

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