Exam 18: Dna Technology: Making and Using Genetically Altered Organisms, and Other Applications

Figure 18.1 - Answer the question using the accompanying figure. Of the fragments labeled A-D, which is closest to the positive electrode?

During DNA cloning, why is the DNA of interest inserted into the middle of the lacZ⁺coding sequence in the plasmid vector?

Figure 18.1 - Answer the question using the accompanying figure. What does letter E represent?

Recombinant DNA is ____.

What is another name for a short tandem repeat (STR) sequence?

Germline and somatic gene therapy are used to treat human genetic diseases.

Restriction enzyme-generated DNA fragments of different lengths from the same region of the genome are called ____.

The protein-coding region of a eukaryotic gene ____.

When cloning DNA into bacteria, the ____.

A researcher wants to insert a DNA fragment into a plasmid. Which enzyme will join the two DNA molecules together?

An organism whose genome has been modified to change a genetic trait or traits by using traditional breeding techniques, classical genetic approaches, or modern molecular manipulation techniques is called a ____.

Reverse-transcriptase PCR ____.

Restriction fragment length polymorphisms (RFLPs) are used to ____.

Explain the ethical concerns with germline gene therapy in humans.

The vectors used in somatic gene therapy are mostly _____.

A researcher is interested in studying gene X, but not gene Y, in Drosophila . What does she need to amplify only gene X from the Drosophila genome?

Reverse transcriptase creates a single-stranded DNA molecule that is complementary to an mRNA template.

Copies of recombinant DNA are produced in a genetically engineered organism by ____.

If a restriction endonuclease cuts a linear DNA three times, how many fragments should there be after the DNA is subjected to electrophoresis through an agarose gel?

Bioinformatics combines the fields of ____ to extract information from biological data.