Exam 18: Dna Technology: Making and Using Genetically Altered Organisms, and Other Applications

Figure 18.1 - Answer the question using the accompanying figure. Of the fragments labeled A-D, which is closest to the negative electrode?

Plasmid cloning vectors generally contain genes for which two traits?

Crops that are resistant to the herbicide Roundup ____.

How is a DNA fragment containing a gene of interest generated for cloning?

DNA fingerprinting is limited to human studies.

Genetically altered organisms are ____.

Discuss the reasons why cloning transgenic animals may be preferable over traditional breeding. What are some of the problems with animal cloning?

Bacteria transformed with plasmids are plated on growth medium containing ampicillin and X-gal. White colonies on the plate contain bacteria with a ____ plasmid, meaning the lacZ⁺gene is ____.

Which feature of a plasmid cloning vector is necessary for the plasmid to be replicated inside a host cell?

Why are numerous replication cycles - each performed at three difference temperatures - a necessary part of a PCR reaction?