Exam 18: Dna Technology: Making and Using Genetically Altered Organisms, and Other Applications

β-galactosidase is used in bacterial screening assays for visualization of colonies that contain recombinant DNA. What is its substrate?

In which situation would gene cloning occur?

Agarose gel electrophoresis separates DNA molecules according to their ____.

When DNA fragments are sealed together by DNA ligase, the process is referred to as ____.

Transgenic organisms contain extra copies of their own genes.

What is the natural function of restriction endonucleases?

Which statement is accurate regarding DNA technologies?

Gene therapy is used to treat many diseases.

What enables a PCR-amplified gene to be inserted into a cloning vector?

What is the main difference between germline gene therapy and somatic gene therapy?

Gel electrophoresis can only be used to analyze DNA samples.

Plasmids are ____.

The characterization of whole genomes, including their structures (sequences), functions, and evolution is called ____.

Some restriction endonucleases cut DNA in such a way that short, single-stranded regions known as ____ ends are created.

Figure 18.2 - Answer the question using the accompanying figure. The figure shows the alleles present at an STR locus in two adults and four juveniles. Which of the juvenile(s) could be offspring of the adults?

How do scientists determine the annealing temperature used in PCR reactions?

What molecular tool has applications like correcting gene mutations, creating specific mutations, knocking out genes, inserting genes, engineering animals for use in human organ transplants, engineering crops and livestock to improve yields, and developing animal models of human genetic diseases?

A plasmid cloning vector and DNA fragments containing the XYZ gene, each digested with the same restriction enzyme, are mixed with DNA ligase. Which product(s) will result?

In agarose gel electrophoresis, DNA is visualized by ____.

Restriction endonucleases break ____ bonds.