Exam 19: Analyzing and Engineering Genes

The restriction enzymes BamHI and BclI cut at the points indicated by arrows: If one sample of DNA was cut with BamHI and another with BclI,and these two samples were mixed and treated with DNA ligase,what would occur?

Plasmids are used as vectors in both plant and bacterial genetic engineering.However,there is a major difference in the fate of genes introduced into bacteria on most bacterial plasmids and into plants on Ti plasmids.What is this difference?

To create a viral vector for delivery of genes into mammalian cells,the virus must be engineered to

The final step in a DNA sequencing reaction is to run the DNA fragments on a gel.What purpose does this serve?

In a single PCR cycle consisting of 15 seconds at 94°C,30 seconds at 50°C,and 1 min at 72°C,what is happening in the step run at 50°C?

Which type of disorder is most difficult to correct by gene therapy?

Imagine that you've prepared a Southern blot and probed it using a single probe.You observe 8 different bands after autoradiography,with one band being very dark and the other 7 being significantly lighter.What is suggested by these results?

If you are a human geneticist looking for an ideal population in which to map disease genes,what characteristic would you want this population to possess?

The areas that have received the greatest attention in genetically modified plants are

If mRNAs could be ligated and replicated within plasmids,what enzyme commonly used in recombinant DNA technology would no longer be needed?

When constructing a cDNA library from a particular organism,the choice of tissue is ________,whereas when constructing a genomic DNA library from the same organism,the choice of tissue is ________.

Imagine that you've isolated the complete human growth hormone gene directly from the human genome.After running through all the steps described in Chapter 19 for cloning and gene expression in bacteria,you find that no human growth hormone is expressed.What is the most likely explanation?

Why did the researchers wear protective clothing and note so emphatically that they did so?

Which of following sequences is most likely to be cut by a restriction enzyme?

One example of an innovation from an unexpected source comes from studies of tumor-like plant growths.What information did the study of plant tumors provide that was critical for plant genetic engineering?

Which of the following is in the correct order for one cycle of PCR?

What is a primary difference between PCR and traditional cloning procedures such as those used to clone the human growth hormone gene?

A bacterial cell that has taken up plasmid DNA is

Gene therapy requires

Imagine that you compare two DNA sequences found in the same location on homologous chromosomes.On one of the homologs,the sequence is AACTACGA.On the other homolog,the sequence is AACTTCGA.Within a population,you discover that each of these sequences is common.These sequences